| dc.description.abstract | The research work carried out involves separation and identification of cardio
vascular drugs, anti-diabetic drug and anti-histamine drugs in human plasma
using solid phase extraction (SPE) and high performance liquid chromatography
(HPLC). The cardiovascular drugs studied are amiloride HCl, metoprolol
succinate, hydrochlorothiazide, carvedilol, amlodipine besilate, frusemide,
telmisartan, losartan potassium and olmesartan. The studied anti-diabetic drugs
are metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone,
glimepiride and repaglinide. The studied anti-histamine drugs are phenylephrine
HCl, cetirizine HCl, loratidine HCl, montelukast sodium and ebastine.
The calculated percentage recoveries of the cardiovascular drugs from plasma
indicated that the values of the percentage recoveries of amiloride HCl,
metoprolol succinate, hydrochlorothiazide, carvedilol, amlodipine besilate,
frusemide, telmisartan, losartan potassium and olmesartan were 60, 65, 30, 10,
30, 10, 10, 10, and 100%, respectively. The remaining amounts of these drugs
(bound to plasma proteins) were 40, 35, 70, 90, 70, 90, 90, 90, and 90%,
respectively. The values of retention, separation and resolution factors were
ranged from 0.19-3.40, 1.20-3.60 and 2.43-12.37, respectively. The reported SPE
and HPLC methods were selective, efficient, rugged, economic, eco-friendly and
reproducible for the separation and identification of amiloride HCl, metoprolol
succinate, hydrochlorothiazide, carvedilol, amlodipine besilate, frusemide,
telmisartan, losartan potassium and olmesartan in human plasma.
The percentage recoveries of metformin HCl, vildagliptin, gliclazide, linagliptin,
sitagliptin, pioglitazone, glimepiride and repaglinide were determined by doing
the blank experiments. The intended percentage recoveries of metformin HCl,
vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and
repaglinide in laboratory synthesized samples in water were 80, 82, 77, 87, 83,
85, 86, and 88%, correspondingly. These values in plasma were 22, 20, 21, 19,
16, 12, 10, and 17%, correspondingly. Low values in the plasma samples were
due to the binding of these drugs with plasma proteins. The values of the
retention, separation and resolution factors were ranged from 0.07 to 9.14, 1.44 to
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4.21 and 2.15 to 18.66, correspondingly. The identification of the separated drugs
was determined by running and comparing the retention times of the individual
metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone,
glimepiride and repaglinide molecules, correspondingly. It was observed that
there was no additional peak in the plasma samples, which established the
selectivity of the SPE method. From the results it was concluded that the reported
SPE and UFLC methods were selective, efficient, rugged, economic, ecofriendly
and reproducible for the separation and identification of metformin HCl,
vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and
repaglinide in human plasma.
The percentage recoveries of phenylephrine HCl, cetirizine HCl, loratidine,
montelukast sodium and ebastine were determined by doing the blank
experiments. The intended percentage recoveries of phenylephrine HCl, cetirizine
HCl, loratidine, montelukast sodium and ebastine in laboratory synthesized
samples in water were 80, 78, 85, 94 and 71%, correspondingly. These values in
plasma were 10, 12, 15, 06 and 29%, correspondingly. Low values in the plasma
samples were due to the binding of these drugs with plasma proteins. The values
of the retention, separation and resolution factors were ranged from 2.00 to 11.00,
1.15 to 2.31 and 1.00 to 6.07, correspondingly. The identification of the separated
drugs was determined by running and comparing the retention times of the
individual phenylephrine HCl, cetirizine HCl, loratidine, montelukast sodium and
ebastine molecules, correspondingly. It was observed that there was no additional
peak in the plasma samples, which established the selectivity of the SPE method.
From the results it wa concluded that the reported SPE and UFLC methods were
selective, efficient, rugged, economic, eco-friendly and reproducible for the
separation and identification of phenylephrine HCl, cetirizine HCl, loratidine,
montelukast sodium and ebastine in human plasma.
The reported SPE, HPLC and UFLC methods for the separation and identification
(analyses) of cardiovascular, anti-diabetic and anti-histamine drugs were
selective, efficient, rugged, economic, eco-friendly and reproducible in human
plasma. There was no extra peak in plasma samples, which confirmed no drugdrug
interaction for the reported drugs. Besides, the absence of any new peak
established no metabolic product of these drugs in human plasma. The separation
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and identification of these drugs are reported first time so far. The developed
SPE, HPLC and UFLC methods were applied successfully for monitoring these
drugs into human plasma. Therefore, SPE, HPLC and UFLC methods can be
applied for the analyses of these drugs in any plasma sample. | en_US |
