DEVELOPMENT OF HPLC AND UFLC METHODS USING FUSED CORE TECHNOLOGY COLUMNS FOR ANALYSES OF SOME DRUGS

Abstract

The research work carried out involves separation and identification of cardio vascular drugs, anti-diabetic drug and anti-histamine drugs in human plasma using solid phase extraction (SPE) and high performance liquid chromatography (HPLC). The cardiovascular drugs studied are amiloride HCl, metoprolol succinate, hydrochlorothiazide, carvedilol, amlodipine besilate, frusemide, telmisartan, losartan potassium and olmesartan. The studied anti-diabetic drugs are metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide. The studied anti-histamine drugs are phenylephrine HCl, cetirizine HCl, loratidine HCl, montelukast sodium and ebastine. The calculated percentage recoveries of the cardiovascular drugs from plasma indicated that the values of the percentage recoveries of amiloride HCl, metoprolol succinate, hydrochlorothiazide, carvedilol, amlodipine besilate, frusemide, telmisartan, losartan potassium and olmesartan were 60, 65, 30, 10, 30, 10, 10, 10, and 100%, respectively. The remaining amounts of these drugs (bound to plasma proteins) were 40, 35, 70, 90, 70, 90, 90, 90, and 90%, respectively. The values of retention, separation and resolution factors were ranged from 0.19-3.40, 1.20-3.60 and 2.43-12.37, respectively. The reported SPE and HPLC methods were selective, efficient, rugged, economic, eco-friendly and reproducible for the separation and identification of amiloride HCl, metoprolol succinate, hydrochlorothiazide, carvedilol, amlodipine besilate, frusemide, telmisartan, losartan potassium and olmesartan in human plasma. The percentage recoveries of metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide were determined by doing the blank experiments. The intended percentage recoveries of metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide in laboratory synthesized samples in water were 80, 82, 77, 87, 83, 85, 86, and 88%, correspondingly. These values in plasma were 22, 20, 21, 19, 16, 12, 10, and 17%, correspondingly. Low values in the plasma samples were due to the binding of these drugs with plasma proteins. The values of the retention, separation and resolution factors were ranged from 0.07 to 9.14, 1.44 to iii 4.21 and 2.15 to 18.66, correspondingly. The identification of the separated drugs was determined by running and comparing the retention times of the individual metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide molecules, correspondingly. It was observed that there was no additional peak in the plasma samples, which established the selectivity of the SPE method. From the results it was concluded that the reported SPE and UFLC methods were selective, efficient, rugged, economic, ecofriendly and reproducible for the separation and identification of metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide in human plasma. The percentage recoveries of phenylephrine HCl, cetirizine HCl, loratidine, montelukast sodium and ebastine were determined by doing the blank experiments. The intended percentage recoveries of phenylephrine HCl, cetirizine HCl, loratidine, montelukast sodium and ebastine in laboratory synthesized samples in water were 80, 78, 85, 94 and 71%, correspondingly. These values in plasma were 10, 12, 15, 06 and 29%, correspondingly. Low values in the plasma samples were due to the binding of these drugs with plasma proteins. The values of the retention, separation and resolution factors were ranged from 2.00 to 11.00, 1.15 to 2.31 and 1.00 to 6.07, correspondingly. The identification of the separated drugs was determined by running and comparing the retention times of the individual phenylephrine HCl, cetirizine HCl, loratidine, montelukast sodium and ebastine molecules, correspondingly. It was observed that there was no additional peak in the plasma samples, which established the selectivity of the SPE method. From the results it wa concluded that the reported SPE and UFLC methods were selective, efficient, rugged, economic, eco-friendly and reproducible for the separation and identification of phenylephrine HCl, cetirizine HCl, loratidine, montelukast sodium and ebastine in human plasma. The reported SPE, HPLC and UFLC methods for the separation and identification (analyses) of cardiovascular, anti-diabetic and anti-histamine drugs were selective, efficient, rugged, economic, eco-friendly and reproducible in human plasma. There was no extra peak in plasma samples, which confirmed no drugdrug interaction for the reported drugs. Besides, the absence of any new peak established no metabolic product of these drugs in human plasma. The separation iv and identification of these drugs are reported first time so far. The developed SPE, HPLC and UFLC methods were applied successfully for monitoring these drugs into human plasma. Therefore, SPE, HPLC and UFLC methods can be applied for the analyses of these drugs in any plasma sample.